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KMID : 1123920020160020348
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 2 p.348 ~ p.352
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Screening of Cytotoxicity and Antimicrobial Effects of Extracts from Atractylodes macrocephala Koidz
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Choi Eun-Young
Oh Hyun-Joo
Park Nang-Kyu
Chun Hyun-Ja
Ahn Jong-Woong
Jeon Byung-Hoon
Han Du-Seok
Lee Hyun-Ok
Baek Seung-Hwa
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Abstract
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This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents
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KEYWORD
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A. macrocephala Koidz., MTT assay, Cytotoxic effects, Antimicrobial activities
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